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Test Code LEGRP Legionella species, Molecular Detection, PCR, Varies

Important Note

This test is only orderable by the laboratory.  To request this test in EPIC, please order LAB001 MAYO MISCELLANEOUS TEST.  Fill out appropriate prompts and request the Mayo test code listed above.

If you need further assistance, please contact the laboratory at 406-414-5010.

Useful For

Sensitive and rapid diagnosis of pneumonia caused by Legionella species

 

The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies

Specimen Required


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Legionella DNA is unlikely.

 

Specimen Type: Lower respiratory

Sources: Bronchoalveolar lavage, bronchial aspirate/brushing/lavage/washing, tracheal/endotracheal secretions/aspirate, sputum Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Fresh tissue or biopsy

Sources: Lung, pleura, heart valve, pericardium

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)  - approximately the size of a pencil eraser

Collection Instructions: Aseptically collect a 1 to 2 cm(3) piece of tissue whenever possible

 

Specimen type: Fluid

Sources: Pericardial, pleural, chest, chest tube drainage, thoracentesis, empyema

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87801

Reject Due To

Tissue in formalin, formaldehyde, or acetone
Formalin-fixed paraffin-embedded (FFPE) block
Reject

Method Name

Rapid Polymerase Chain Reaction (PCR)

Report Available

3 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.