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Test Code LAB829 Iron and TIBC

Useful For

Screening for anemia and chronic iron overload diseases, particularly hereditary hemochromatosis

Profile Information

Test Code Test Name Methodology

Available

Separately

Always

Performed

LAB94 Iron, Total Colorimetric FerroZine Yes Yes
N/A Unsaturated Iron-Binding Capacity (UIBC) Colorimetric FerroZine No Yes
N/A Total Iron-Binding Capacity (TIBC) Calculated Yes Yes
N/A % Iron Saturation Calculated No Yes

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Plasma or serum
Container/Tube: Light-green top (lithium heparin gel), red top, or gold top
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL

Collection Instructions:

  1. Specimens should be collected in the morning to avoid low results due to

    diurnal variation. 

  2. Allow serum samples to clot. 
  3. Separate serum or plasma from clot within 1 hour.

Specimen Rejection: Hemolyzed samples may be rejected.

Specimen Transport Temperature

Ambient/Refrigerated

Specimen Stability

Specimen Type Temperature Time
Plasma or Serum Ambient 4 days
  Refrigerated 7 days

Reference Values

IRON

Male: 44-179 ug/dL

Female: 31-172 µg/dL

 

UIBC

112-347 ug/dL

 

TIBC

Adult male: 260-400 ug/dL

Adult female: 260-445 ug/dL

 

% Iron Saturation (calculated)

20%-50% saturation

Additional Information

In the presence of high ferritin concentrations > 1200 µg/L the assumption that serum iron is almost completely bound to transferrin is not valid anymore. Therefore, such iron results should not be used to calculate Total Iron Binding Capacity (TIBC) or percent transferrin saturation (% SAT).

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

83540-IRON

83550-TIBC