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HelpTest Code LAB600 Protein Serum Electrophoresis, Reflex to Immunofixation
Useful For
- Diagnosis of monoclonal gammopathies, when used in conjunction with urine monoclonal studies
- Monitoring patients with monoclonal gammopathies
Profile Information
| Test Code | Test Name | Methodology |
Available Separately |
Always Performed |
|---|---|---|---|---|
| LAB118 | Total Protein, Serum | Bichromatic End Point | Yes | Yes |
| N/A | Protein Electrophoresis, Serum | Agarose Gel Electrophoresis | Yes | Yes |
Testing Algorithm: If a discrete electrophoresis band is identified, serum immunofixation testing will be reflexed, if necessary, at an additional charge.
Reflex Tests
| Test Code | Test Name | Methodology |
Available Separately |
Always Performed |
|---|---|---|---|---|
| LAB174 (LAB ONLY) | Immunofixation, Serum | Immunofixation | No | No |
Performing Laboratory
Bozeman Health Outpatient Services at North 19th
Specimen Requirements
Specimen Type: Serum
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 1 mL
Collection Instructions:
1. Indicate if multiple myeloma is suspected.
2. Let the specimen clot completely before centrifugation.
Specimen Rejection:
1. Hemolyzed specimens.
2. Specimen types other than serum.
Specimen Transport Temperature
Ambient<8 hours/Refrigerated>8 hours
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Ambient | 8 hours |
| Refrigerated | 7 days | |
| Frozen | 1 month |
Reference Values
| Test Component | Normal Range |
|---|---|
| Albumin SPEP | 3.8-5.5 g/dL |
| Alpha-1-globulin | 0.1-0.3 g/dL |
| Alpha-2-globulin | 0.5-1.0 g/dL |
| Beta-globulin | 0.5-1.0 g/dL |
| Gamma-globulin | 0.5-1.5 g/dL |
| M-Spike | 0.0-0.0 g/dL |
| Total Protein | 6.4-8.2 g/dL |
An interpretive comment is provided with the report. If reflexed, an immunofixation interpretive comment is also provided with the report.
Additional Information
Immunofixation will not be performed if performed on a previous specimen, unless otherwise specified.
Day(s) Test Set Up
Monday, Wednesday, and Friday
Test Classification and CPT Coding
84155-Total Protein, serum
84165-Electrophoresis, protein
86334-Immunofixation (if appropriate)