Test Code LAB54 Calcium, Ionized, Whole Blood, Plasma or Serum
Useful For
Determining the “physiologically active” or free calcium level in patients with altered proteins (chronic renal failure, malabsorption, multiple myeloma, and nephrotic syndrome), and in disturbances of acid-base metabolism.
Methodology
Ion-Selective Electrode (ISE)
Performing Laboratory
Bozeman Health Laboratory Services
Specimen Requirements
Submit only 1 of the following specimens:
Specimen Type: Whole blood
*NOTE: Only draw whole blood sample if specimen can be processed within 30 minutes
Container/Tube: Dark-green top (sodium heparin) or light-green top (lithium heparin)
Specimen Volume: Full tube
Collection Instructions:
- Keep specimen anaerobic. Do not transfer or uncap tube.
- Send to laboratory within 30 minutes.
Specimen Type: Plasma or serum
Container/Tube: Light-green top (lithium heparin gel), green top Microtainer, gold top, or gel Microtainer
Specimen Volume: Full tube
Collection Instructions:
- Allow blood to clot.
- Centrifuge with stopper in place for 10 minutes at 3,200 rpm to ensure gel barrier separates serum from cells.
- Keep specimen anaerobic. Do not transfer or uncap tube.
- Label specimen as plasma or serum.
Specimen Transport Temperature
Whole blood: Ambient <30 minutes
Plasma or serum: Refrigerated
Specimen Stability
Specimen Type | Temperature | Time |
---|---|---|
Whole blood | Ambient | 30 minutes |
Plasma or Serum | Refrigerated, anaerobic (capped) | 5 days |
Reference Values
Age | Normal Range | Critical Values |
---|---|---|
0-6 months | 1.00-<1.50 mmol/L | >1.50 mmol/L |
≥ 6 months | 1.12-1.32 mmol/L | >1.50 mmol/L |
Critical value (automatic call-back): >1.50 mmol/L
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
82330