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Test Code LAB308 Factor IX (9) Assay

Specimen Type/Requirements

Blue top (Sodium Citrate) tube - Double Spun Platelet Poor Plasma

 

See Coagulation Testing and Platelet Poor Plasma Preparation

 

This test requires its own separate frozen aliquot.  The aliquot tube must be from an appropriately filled 3.2% Sodium Citrate tube to acquire the 9:1 blood to anticoagulant ratio that is critical for valid results.

 

Platelet poor plasma should be frozen within 2 hours of collection.  Double spun (platelet poor plasma) is critical for accurate results.

 

Patient must not be receiving Heparin or Coumadin Therapy.

 

Test is affected by moderate hemolysis.  Test is not affected by lipemia.

Specimen Volume

 Preferred Volume     1.0 mL   
 Minimum Volume     0.5 mL   

 

Stability/Transport

 Room Temperature     2 hours     
 Refrigerated     4 hours     
 Frozen     1 month     Preferred for transport   

 

Performed Test Frequency

Monday through Friday

Methodology

Activated Partial Thromboplasting Clot-Based Assay

CPT

85250

Performing Lab

Sanford Laboratories Sioux Falls

Useful For

Diagnosing deficiencies, particularly Hemophilia B (Christmas Disease); Assessing the impact of liver disease on hemostasis; Investigation of a prolonged Activated Partial Thromboplastin Time.

 

Ordering Information:  

Note: If the patient is on any of the following medications for Hemophilia B (see list below).  This test (BLOD0662) is not appropriate and should not be ordered.  Order instead, Mayo Chromogenic Factor IX Activity Assay (BLOD1654).

   Medications Requiring Chromogenic Assays (BLOD1654)*   
Alprolix
Idelvion
Rebinyn
Refixia

*There may be other extended half-life medication treatments for Hemophilia B not included in the list that may require the Chromogenic Factor IX Activity Assay (BLOD1654).