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HelpTest Code LAB3001 Rh Immune Globulin Screen and Dose
Useful For
Determining if a patient is a candidate for Rhogam and calculating the correct dose of Rhogam needed. Rhogam is administered to Rh-negative mothers who deliver Rh-positive (or unknown Rh) babies as an effective means of preventing Rh-alloimmunization. In most instances, D-negative mothers delivering D-positive infants can be protected from producing anti-D by the administration of Rhogam within 72 hours of delivery. A 300 ug dose of Rhogam is sufficient to suppress Rh immunization for no more than 30 mL of fetal whole blood that has entered the maternal circulation.
Methodology
Capture solid phase automation and manual blood banking methods
Profile Information
| Test Code | Test Name | Always Performed |
|---|---|---|
| LAB895 | ABO and Rh Type, Blood | Yes |
| LAB896 | Fetal Screen | Yes |
Testing Algorithm:
If the fetal screen is positive, a Kleihauer-Betke test will automatically be reflexed.
Reflex Tests
| Test Code | Test Name | Available Separately | Always Performed |
|---|---|---|---|
| LAB762 | Kleihauer-Betke, Blood | Yes | No |
Performing Laboratory
Bozeman Health Laboratory Services
Specimen Requirements
Specimen Type: EDTA whole blood
Container/Tube: Purple top or pink top (K2 EDTA)
Specimen Volume: 6 mL
Specimen Minimum Volume: 3 mL
Collection Instructions: Collect blood from mother at least 1 hour after delivery of all products of conception and collect as soon as possible thereafter. Do not centrifuge. Forward unprocessed whole blood promptly. Rhogam must be given within 72 hours of delivery.
Specimen Rejection:
1. Gel tubes will be rejected.
2. Specimen must be labeled properly.
3. This test is affected by gross hemolysis.
Specimen Transport Temperature
Ambient <24 hours/Refrigerated >24 hours
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Ambient | 24 hours |
| Refrigerated | 48 hours |
Reference Values
Type: A, B, O, AB, A2
Rh: Positive or Negative
Fetal Screen: Negative (One dose of Rhogam considered sufficient)
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
86900-ABO
86901-Rh type
85461-Fetal screen