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HelpTest Code LAB278 Antibody Screen (Indirect Coombs), Blood
Useful For
Detecting the presence of irregular antibodies in patient’s serum
Methodology
Capture solid phase automation and manual blood banking methods
Performing Laboratory
Bozeman Health Laboratory Services
Specimen Requirements
Specimen Type: Whole blood
Container/Tube: Pink top preferred (K2 EDTA) or Purple top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Do not centrifuge. Forward unprocessed whole blood promptly.
Specimen Rejection: Gel tubes are not acceptable. Specimen must be labeled properly. This test is affected by gross hemolysis.
Note: If specimen being drawn may be used for a possible transfusion, refer to test LAB276 TYPE AND SCREEN for banded specimen collection instructions and blood product transfusion instructions.
Specimen Transport Temperature
Ambient <24 hours/Refrigerated >24 hours
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Ambient | 24 hours |
| Refrigerated | 4 days | |
| EDTA plasma | Frozen | 60 days |
Reference Values
Negative (reported as positive or negative)
If screen is positive, then antibody identification, antigen screen, and direct Coombs will be performed at an additional charge. If these additional tests are not desired, the ordering physician/provider must indicate “No reflex test is desired” on request form.
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
86850-Antibody screen
86870-Antibody identification (if appropriate)
86880-Antiglobulin (direct Coombs) (if appropriate)
86905-Each red cell antigen typing (if appropriate)