Test Code LAB250 Gram Stain
Useful For
- Identifying microorganisms in normally sterile body fluids
- Guiding initial antimicrobial therapy
Methodology
Conventional Gram Stain procedure. Body fluids will be concentrated by cytospin.
Performing Laboratory
Bozeman Health Laboratory Services
Specimen Requirements
Specimen source is required.
Specimen Type: Fluid
Container/Tube: Syringe with needle removed, anaerobic transport vial, or screw-capped, sterile container
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL
Collection Instructions:
1. Using needle and syringe, aspirate fluid.
2. Transfer aspirated fluid to vial or container.
3. If submitting specimen in syringe, remove needle and cap syringe.
4. Label vial/container/syringe with patient’s name (first and last), date and actual time of collection, and type of specimen.
Specimen Type: Solid tissue
Container/Tube: Anaerobic transport vial or screw-capped, sterile container
Specimen Volume: Adequate amount
Collection Instructions:
1. Aseptically collect material.
2. Transfer material to vial or container.
3. Label vial/container with patient’s name (first and last), date and actual time of collection, and type of specimen.
Specimen Type: Varies
Container/Tube: ESwab, Copan swab
Specimen Volume: Entire specimen
Collection Instructions:
1. Prepare site and obtain specimen.
2. Return swab to transport tube and cap tightly. If using an ESwab, break the swab shaft at the breakpoint indicated by the colored line and discard the remaining shaft.
3. Label tube with patient’s name (first and last), date and actual time of collection, and type of specimen.
Specimen Rejection: Blood
Specimen Transport Temperature
Ambient
Specimen Stability
Specimen Type | Temperature | Time |
---|---|---|
Anaerobic Transport Vial | Ambient | 72 hours |
Capped Syringe | Ambient | 2 hours |
ESwab | Ambient | 48 hours |
Copan Swab | Ambient | 24 hours |
Reference Values
No organisms seen
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
87015-Cytospin concentration (if indicated)
87205-Gram stain