Test Code LAB2424 Yeast Culture
Useful For
Recovery and identification of Yeast
Methodology
Conventional Biochemical Identification Methods
Performing Laboratory
Bozeman Health Laboratory Services
Specimen Requirements
Submit only one of the following specimens:
Specimen Type: Cervix, endocervix, vaginal
Container/Tube: ESwab
Specimen Volume: Adequate amount
Collection Instructions:
- Do not use lubricant on speculum.
- Remove excess mucus, blood, and/or examination lubricants from cervix.
- Insert swab and rotate swab for 15 to 30 seconds to ensure adequate sampling.
- Return swab to transport tube.
- Label tube with patient’s name (first and last), date and actual time of collection, and type of specimen.
*Note: Refer to Affirm testing (NBLD0373 VAGINITIS PROBE) to rule out Candida yeast infections.
Specimen Type: Urethra
Container/Tube: Male Urethral Swab
Specimen Volume: Adequate amount
Collection Instructions:
- Patient should not have urinated for at least 1 hour prior to specimen collection.
- Insert small-tipped specimen swab 2 cm to 4 cm into urethra and rotate swab for 3 to 5 seconds to ensure adequate sampling.
- Return swab to transport tube.
- Label tube with patient’s name (first and last), date and actual time of collection, and type of specimen.
Specimen Type: Sputum-expectorated
Container/Tube: Tightly-sealing, screw-capped, sterile container
Specimen Volume: Adequate amount
Collection Instructions:
- Patient should not have eaten prior to collection.
- Specimen should be a single, first-morning, “deep-cough” sputum specimen.
- Have patient remove dentures (if applicable).
- Instruct patient to brush his/her teeth and/or rinse mouth well with water.
- Instruct patient to take a deep breath, hold it momentarily, and cough deeply and vigorously into container (send entire volume).
- Label container with patient’s name (first and last), date and actual time of collection, and type of the specimen.
Specimen Rejection: Nasal secretions or saliva will be rejected.
Specimen Type: Wound, Deep or Superficial, Abscess
Container/Tube: ESwab collection kit
Specimen Volume: Adequate amount
Collection Instructions:
- Prepare site, wash wound margins well with antiseptic soap, and rinse with sterile saline..
- Break surface of wound. Pass ESwab deep into abscess and sample lesion’s advancing edge, taking care to avoid contamination with other normal flora.
- Aseptically unscrew and remove the cap from the tube.
- Insert the ESwab into the tube and break the swab shaft at the breakpoint indicated by the colored line marked on the swab shaft. Discard the remaining broken portion of the swab shaft. Replace cap on the tube and secure tightly.
- Label tube with patient’s name (first and last), date, actual time of collection, and type of specimen.
Specimen Type: Tissue or Body Fluid: abdominal, ascites, bile, pericardial, peritoneal, pleural fluid, synovial fluid, serous fluid, or closed abscess.
Container/Tube: Screw-capped, sterile container
Acceptable: Syringe (needle removed)
Specimen Volume: Entire specimen
Collection Instructions:
- Disinfect overlaying skin with chlorhexidine gluconate.
- Using needle and syringe, aspirate fluid.
- Transfer entire volume of aspirated fluid to vial, container, or leave in syringe.
- If submitting specimen in syringe, remove needle and cap syringe.
- Label vial/container/syringe with patient’s name (first and last), date and actual time of collection.
Specimen Type: Spinal fluid
Container/Tube: Screw-capped, sterile CSF tube
Specimen Minimum Volume: 1 mL
Collection Instructions:
- Label tube(s) with patient’s name (first and last), date and actual time of collection, and in order of collection 1, 2, 3, etc.
- Maintain sterility and forward promptly. Do not send by pneumatic tube. This test will be performed on tube #2 or #3 of tubes submitted from spinal fluid collection.
*For CSF, also order test: NBLD0334 CRYPTOCOCCUS ANTIGEN SCREEN, CSF
Additional Information
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This test will include a Gram stain on appropriate sources.
-
Susceptibilities are available upon request, for an additional charge.
Specimen Transport Temperature
Ambient
Specimen Stability
Specimen Type | Temperature | Time |
---|---|---|
ESwab | Ambient |
48 hours |
NP Swab | Ambient | 24 hours |
CSF | Ambient | 1 hour |
Syringe | Ambient | 2 hours |
Bone or Body Fluid in Sterile Container | Ambient | 1 hour |
Sputum, BAL, Tracheal Aspirate, Bronchial Washings | Ambient | 2 hours |
Refrigerated | 24 hours |
Reference Values
No Yeast isolated
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
87102-Yeast culture