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Test Code LAB1749 Manual Differential, Blood

Useful For

Visually examining cell morphology when abnormal blood counts, abnormal cell morphology, or a potential disease process is suspected

Methodology

Light Microscopy

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA) or lavender top (EDTA) Microtainer

Specimen Volume: 4 mL

Pediatric Volume: 1 full Microtainer

Collection Instructions: If there is a delay in transport of >24 hours, refrigerate specimen and send specimen refrigerated.

Specimen Rejection: Unrefrigerated specimen> 24 hours old. Refrigerated specimen >48 hours old.

Specimen Transport Temperature

Ambient <24 hours/Refrigerated <48 hours

Specimen Stability

 

Specimen Type Temperature Time
Whole blood (EDTA) Ambient 24 hours
  Refrigerated 48 hours
Stained Smear Ambient (storage at BDH) 1 week
  Ambient, sealed & coversliped Indefinitely

 

Additional Infomation

  • Testing includes WBC differential, platelet estimate, and WBC and RBC morphology.
  • This test is not usually performed without a CBC.

  • Absolute count (K/uL)= % of cell type in differential (in decimal form) x total WBC count

Example:

% Lymphocytes: 50%

Total WBC count: 4.3 K/uL

Absolute Lymphocyte Count (K/uL)= 0.50 x 4.3 = 2.15 K/ul

Reference Values

Reference Values established for ≥ 18 years of age:

 

Component % Normal Range Absolute Normal Range
WBC count   3.2 - 10.8 K/uL
Neutrophils 42.0% - 75.0% 1.4 - 6.5 K/uL
Lymphocytes 20.0% - 51.0% 1.2 - 3.4 K/uL
Monocytes 3.0% - 12.7% 0.2 - 1.0 K/uL
Eosinophils  0.0% - 10.0% 0.0 - 0.7 K/uL
Basophils 0.0% - 4.0% 0.0 - 0.5 K/uL
Bands 0.0% - 10.0% 0.0 - 1.0 K/uL

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

85007