Test Code LAB1729 Flow Cytometry, Leukemia/Lymphoma Phenotyping
Useful For
Aiding in the diagnosis of leukemia and lymphoma, and for post treatment follow up
Methodology
Flow Cytometry
Performing Laboratory
Yellowstone Pathology Institute
Specimen Requirements
Specimen Type: Bone Marrow Aspirate
Container/Tube:
Preferred: EDTA (lavender top)
Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)
Specimen Volume: 2 mL
Specimen Minimum Volume: 1 mL
Collection Instructions:
- Invert several times to mix bone marrow.
- Send specimen at ambient temperature in original container.
- Include a YPI requisition and copy of a recent CBC report.
Specimen Type: Bone Marrow Core
Container/Tube: RPMI
Specimen Volume: 2 cm core length
Specimen Minimum Volume: 1 cm core length
Collection Instructions:
- Send RPMI specimen at ambient temperature.
- Include a YPI requisition and copy of a recent CBC report.
Specimen Type: Peripheral Blood
Container/Tube:
Preferred: EDTA (lavender top)
Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)
Specimen Volume: 2 mL
Specimen Minimum Volume: 1 mL
Collection Instructions:
- Invert several times to mix blood.
- Send specimen at ambient temperature in original container.
- Include a YPI requisition and copy of a recent CBC report.
Specimen Type: Fresh Tissue
Container/Tube: RPMI
Specimen Minimum Volume: 2 pieces of tissue 0.2 cm3
Collection Instructions:
- Send RPMI specimen at ambient temperature.
- Include a YPI requisition and copy of a recent CBC report.
Specimen Type: Body Fluid/Fine Needle Aspirate
Container/Tube: RPMI
Specimen Volume: Equal parts RPMI and specimen volume
Collection Instructions:
- Send RPMI specimen at ambient temperature.
- Include a YPI requisition and copy of a recent CBC report.
Specimen Rejection:
1. Unlabeled specimens.
2. Specimens collected in wrong container/tube.
3. Specimens retained past viability.
Specimen Transport Temperature
Ambient
Specimen Stability
Specimen Type | Temperature | Time |
---|---|---|
Varies | Ambient |
48-72 hours* |
*For optimal specimen recovery, transport specimen to YPI immediately.
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
88184 Leukemia/Lymphoma Phenotyping (1 marker)
88187 Leukemia/Lymphoma Phenotyping (2-8 markers)
88188 Leukemia/Lymphoma Phenotyping (9-15 markers)
88189 Leukemia/Lymphoma Phenotyping (16+ markers)