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Test Code LAB1729 Flow Cytometry, Leukemia/Lymphoma Phenotyping

Useful For

Aiding in the diagnosis of leukemia and lymphoma, and for post treatment follow up

Methodology

Flow Cytometry

Performing Laboratory

Yellowstone Pathology Institute

Specimen Requirements

Specimen Type: Bone Marrow Aspirate

Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions:

  1. Invert several times to mix bone marrow.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Bone Marrow Core

Container/Tube: RPMI

Specimen Volume: 2 cm core length

Specimen Minimum Volume: 1 cm core length

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Peripheral Blood

Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions:

  1. Invert several times to mix blood.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Fresh Tissue

Container/Tube: RPMI

Specimen Minimum Volume: 2 pieces of tissue 0.2 cm3

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Body Fluid/Fine Needle Aspirate

Container/Tube: RPMI

Specimen Volume: Equal parts RPMI and specimen volume

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

 

Specimen Rejection:

1.  Unlabeled specimens.

2.  Specimens collected in wrong container/tube.

3.  Specimens retained past viability.



Specimen Transport Temperature

Ambient

Specimen Stability

Specimen Type Temperature Time
Varies Ambient

48-72 hours*

*For optimal specimen recovery, transport specimen to YPI immediately.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

88184 Leukemia/Lymphoma Phenotyping (1 marker)

88187 Leukemia/Lymphoma Phenotyping (2-8 markers)

88188 Leukemia/Lymphoma Phenotyping (9-15 markers)

88189 Leukemia/Lymphoma Phenotyping (16+ markers)