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Test Code LAB168 Bilirubin: Total, Direct, and Indirect

Useful For

Detecting hemolytic anemia, diagnosing liver disease, and evaluating degrees of jaundice.

Profile Information

Test Code Test Name Methodology

Available

Separately

LAB52

Bilirubin Direct (Conjugated)

Photometric, Diazotized Sulfanilic Acid (Jendrassik-Grof) Yes
LAB50 Bilirubin Total

 

Colorimetric Diazo

Yes
N/A Bilirubin Indirect (Unconjugated) Calculation No

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Plasma or serum
Container/Tube: Light-green top (lithium heparin gel), red top, or gold top
Specimen Volume: 2 mL
Specimen Minimum Volume: 0.5 mL 

Specimen Pediatric Volume: 1 microtainer (amber)

Collection Instructions: Protect specimen from light.

Specimen Rejection:  Hemolyzed samples may be rejected.

Specimen Transport Temperature

Ambient<24 hours/Refrigerated>24 hours

Specimen Stability

Specimen Type Temperature Time
Plasma or Serum Ambient 1 day
  Refrigerated 7 days
  Frozen 6 months

Note: Stability values are only valid if care is taken to prevent exposure to light. 

Reference Values

Component Age Break Normal Range Critical Values
Neonatal Bilirubin, Total 0-1 days of life 0.0-4.8 mg/dL 6.4 and higher
  1-2 days of life 0.0-8.8 mg/dL 10.6 and higher
  2-3 days of life 0.0-12.1 mg/dL 13.5 and higher
  3-4 days of life 0.0-14.0 mg/dL 14.5 and higher
  4-30 days of life 0.0-15.5 mg/dL 16.1 and higher
Neonatal Bilirubin, Direct 0-60 days 0.2-1.0 mg/dL >20.0 mg/dL
  ≥ 60 days 0.0-0.3 mg/dL >20.0 mg/dL
Neonatal Bilirubin, Indirect 0-30 days 0.0-11.7 mg/dL  
  ≥ 30 days 0.0-1.0 mg/dL  

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

82247

82248