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Test Code LAB124 Testosterone, Total

Useful For

  • Evaluation of men with symptoms or signs of possible hypogonadism, such as loss of libido, erectile dysfunction, gynecomastia, osteoporosis, or infertility
  • Evaluation of boys with delayed or precocious puberty
  • Monitoring testosterone replacement therapy
  • Evaluation of women with hirsutism, virilization, and oligoamenorrhea
  • Evaluation of women with symptoms or signs of possible testosterone deficiency

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Plasma or Serum
Container/Tube: Light green top (Lithium heparin), gold top or red top
Specimen Volume: 2 mL
Specimen Minimum Volume: 1 mL

Specimen Rejection:

  1. Hemolyzed samples may be rejected.
  2. Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration.

Specimen Transport Temperature

Ambient/Refrigerated

Specimen Stability

 

Specimen Type Temperature Time
Plasma or Serum Ambient 5 days
  Refrigerated 14 days
  Frozen 6 months

Reference Values

Gender Age Normal Range
Male ≤9 yrs 7-29 ng/dL
  10-19 yrs

123-814 ng/dL
  19-40 yrs 303-852 ng/dL
  ≥40 yrs ≥300 ng/dL
Female   9-48 ng/dL

Additional Information

  • Phenylbutazone at therapeutic dosage levels showed interference with this assay (testosterone values increased)
  • Do not use samples from patients under Nandrolone treatment.
  • In isolated cases, elevated testosterone levels can be seen in samples from female patients with end stage renal disease.
  • Implausible elevated testosterone values in women should be verified by an extraction method or a validated LC-MS tandem method.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

84403