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Test Code LAB000 MISC TEST (STATE MEASLES) Measles (Rubeola) Direct Detection by Real Time PCR

Important Note

This test is only orderable by the laboratory.  To request this test in EPIC, please order LAB000 MISCELLANEOUS TEST. Fill out appropriate prompts and request the test "Measles by PCR".

If you need further assistance, please contact the laboratory at 406-414-5010.

Useful For

Determining an active, current infection with the Measles virus during local outbreaks

Methodology

Real Time PCR

Performing Laboratory

Montana State Public Health Laboratory

Specimen Requirements

Collect specimens as soon after the rash as possible. Collection is optimum day 1 through 3 of rash onset.

 

Submit one of the following specimens:

 

Specimen Type: Throat, nasopharyngeal or nasal swab

Collection Container/Tube: Dacron swab in VTM

Submission Container/Tube: Viral transport media


Collection Instructions:

  1. Collect specimen and break off dacron swab into Viral Transport Media.
  2. Label tube with patient’s name, date and actual time of collection, and type of specimen.
  3. If transport will be >24 hours, freeze VTM with swab included at -70° and transport frozen on dry ice. 

 

Specimen Type: Urine

Container/Tube: Clean container

Specimen Minimum Volume: 50 mL

Collection Instructions:

  1. Collect a random urine collection.
  2. Label tube with patient’s name, date and actual time of collection.
  3. Centrifuge urine at 2500xg for 15 minutes at 4° C.
  4. Resuspend sediment in viral transport media.
  5. If transport will be >24 hours, freeze VTM with swab included at -70° and transport frozen on dry ice. 

Note: CSF may be submitted in meningitis/encephalitis-suspect cases with prior consult.  Call the main laboratory at 414-5010.

Specimen Transport Temperature

Refrigerated <24 hours/Frozen >24 hours

Specimen Stability

 

Specimen Type Temperature Time
Swab in Viral Transport Media Refrigerated 24 hours
  Frozen (-70°) 6 months
Urine in Viral Transport Media Refrigerated 24 hours
  Frozen (-70°) 6 months

Reference Values

Negative

Positive results are telephoned to the submitter.

Day(s) Test Set Up

Monday through Friday

Turnaround Time: 1-2 working days

Test Classification and CPT Coding

87798