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Test Code NBLD0379 Fetal Rupture Membrane Screen

Useful For

Detecting the rupture of fetal membranes.  This assay detects the PAMG-1 protein marker of amniotic fluid when present in the vaginal secretions of pregnant women.


Amnisure Rapid Immunochromatographic Assay

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Vaginal secretions

Collection Container/Tube: Swab

Submission Container/Tube: Amnisure Specimen Collection Kit

Specimen Volume: Adequate amount

Collection Instructions:

  1. Take the solvent vial by its cap and shake well to make sure all liquid in the vial has dropped to the bottom. 
  2. Patient should by lying flat on her back.
  3. Hold the provided sterile swab by the middle of the stick and insert into the vagina until the fingers contat the vaginal mucosa, no more than 2-3 inches deep.
  4. Withdraw the swab after 1 minute.
  5. Place the swab into the solvent vial and rinse the swab in the solvent by rotating for 1 minute.  
  6. Remove and discard swab, tightly cap the solvent vial.
  7. Label the solvent vial with patient’s name (first and last), date and actual time of collection, and type of specimen.
  8. Promptly deliver the specimen to the laboratory.  Specimens are only stable at room temperature for 30 minutes.

Specimen Rejection:

1.  Do not test earlier than 6 hours after the removal of any disinfectant solutions or medicines from the vagina.

2.  Placenta previa and performing digital exams prior to sample collection can lead to inaccurate test results.

3.  A significant presence of blood on the swab can cause test malfunction.  In cases of only trace amounts of blood on the swab, the test still functions properly.

Specimen Transport Temperature

Ambient <30 minutes/Refrigerated >30 minutes

Specimen Stability

Specimen Type Temperature Time
Vaginal Secretions in Amnisure solvent vial Ambient 30 minutes
  Refrigerated 6 hours


Reference Values

Negative (No rupture of fetal membranes)


Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding