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Test Code NBLD0300 KOH Prep: Skin, Hair, or Nails

Useful For

Detection of fungi in clinical specimens

Methodology

15% KOH Direct Prep

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen source and suspected fungal species are required.

 

Submit only 1 of the following specimens:

 

Specimen Type: Hair

Container/Tube: Screw-capped, sterile container

Specimen Volume: 10 to 12 hairs

Collection Instructions: Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.

 

Specimen Type: Nails

Container/Tube: Screw-capped, sterile container

Specimen Volume: Adequate amount

Collection Instructions:
1. Wipe nail with 70% alcohol.
2. Scrape outer surface of nail and discard.
3. Collect scraping from deeper, diseased areas of nail, and place in container.
4. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.
 

Specimen Type: Skin scraping

Container/Tube: Screw-capped, sterile container

Specimen Volume: Adequate amount

Collection Instructions:
1. Clean skin with 70% alcohol.
2. Scrape advancing edge of lesion with a sterile glass slide into container.
3. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.

Specimen Transport Temperature

Ambient

Specimen Stability

Specimen Type Temperature Time
Skin, Hair or Nails in sterile container Ambient 7 days

 

Reference Values

Negative: No fungal elements observed

 

Positive: Yeast present, Hyphae present, Yeast and Hyphae present

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

87220