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Test Code NBLD0194 Urinalysis, Reflex to Culture

Useful For

Screening for urinary tract diseases

Methodology

IRIS iQ200 automated microscopy

IR IS AX-4280 automated chemical analysis

Additional Information

This test includes:

  • Automated urine chemistry dipstick
  • Microscopic analysis (regardless of dipstick results).  

A culture will be reflexed if indicated by microscopic results.

 

Culture Reflex Criteria:

A urine culture will be reflexed when two or more of the following parameters are positive:

  • Nitrite
  • Leukocyte Esterase
  • WBC >5/hpf

The following dipstick results will reflex a culture:

Nitrite + and Leukocyte Esterase +
Leukocyte Esterase + and WBC >5/hpf
Nitrite + and WBC>5/hpf
Nitrite +, Leukocyte Esterase + and WBC >5/hpf

 

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Urine

Container/Tube: Sterile container or BD Tiger Top Vacutainer and BD Grey Top Vacutainer

 

 

Specimen Volume: 10 mL

Specimen Minumum Volume: 2 mL sterile cup, 8 mL BD tiger-top, 3 mL BD grey-top vacutainer.  Note minimum/maximum fill lines.

Collection Instructions:

1. Collect a random, first-morning urine collection.

2. Overnight incubation in bladder yields highest bacterial counts.

3. Specimen collected at other times are acceptable.

Specimen Rejection:

1. When sending BD Vacutainer tubes, the lab must receive both a red/yellow tiger top tube and a grey top tube.  Tiger top tubes are suitable for urinalysis only and grey top tubes are suitable for culture only.

2. Urine samples exceeding specimen stability.

3. Urine samples <2 mL in volume. 

4. Urine samples below minimum fill line for BD vacutainers.

 

 

Males

1. Before beginning collection, wash hands with soap and water.

2. Circumcised patients clean beginning of urethra with provided towelette.

3. Uncircumcised patients withdraw foreskin and clean beginning of urethra with provided antiseptic towelette.

4. Urinate a small amount into toilet, then collect a midstream portion of urine into container, void remainder into toilet.

5. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.

 

Females

1. Before beginning collection, wash hands with soap and water.

2. Thoroughly clean urethral area with soap and water or with provided antiseptic towelette.
3. While holding labia apart urinate a small amount into toilet, then collect a midstream portion of urine into container, void remainder into toilet.

4. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.

Specimen Transport Temperature

Ambient <2 hours/Refrigerated >2 hours

Specimen Stability

 

Specimen Type Temperature Time
Urine in Sterile Container Ambient 2 hours
  Refrigerated 48 hours
BD Tiger-top Vacutainer UA Preservative Tube Ambient 72 hours
  Refrigerated 72 hours
BD Grey-top Vacutainer Culture Preservative Tube Ambient 48 hours
  Refrigerated 48 hours

 

Reference Values

Reference values are included with patient’s report.

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

81001-Automated urinalysis w/microscopy