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Test Code LBOR0182 Next-Generation Sequencing (NGS), Acute Myeloid Leukemia

Additional Codes

Mayo Test Code: NGSAM

Useful For

Evaluation of acute myeloid leukemia (AML) at the time of diagnosis, to assist in appropriate classification and prognosis using a 19-gene panel

 

Evaluation to determine if a different gene mutation profile is present at the time of AML relapse

Testing Algorithm

See Targeted Genes Interrogated by Acute Myeloid Leukemia by Next-Generation Sequencing in Special Instructions for a list of the genes and exons targeted by this test.

Method Name

Somatic Mutation Detection by Next-Generation Sequencing (NGS), Hematologic Neoplasms

Specimen Type

Varies


Advisory Information


This test (NGSAM) provides a wider gene mutation analysis for acute myeloid leukemias (AML) and is also appropriate for assessment for myeloid malignancies other than AML.

 

If testing focused more specifically on the gene mutations that are most prevalent and clinically significant in acute myeloid leukemias (AML) is desired, order NGAML / Next-Generation Sequencing (NGS), Acute Myeloid Leukemia, 8-Gene Panel.



Shipping Instructions


Bone marrow and peripheral blood specimens must arrive within 14 days of collection.



Necessary Information


The following information is required:

1. Clinical diagnosis

2. Pertinent clinical history

3. Clinical or morphologic suspicion

4. Date of collection

5. Specimen source



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Bone marrow aspirate (preferred)

Container/Tube:

Preferred: EDTA (lavender top) or ACD (yellow top)

Acceptable: Heparin (Green top)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

Specimen Stability: Ambient (preferred)/Refrigerate

 

Specimen Type: Peripheral blood

Container/Tube:

Preferred: EDTA (lavender top) or ACD (yellow top)

Acceptable: Heparin (green top)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

Specimen Stability: Ambient (preferred)/Refrigerate

 

Specimen Type: Extracted DNA

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions: Label specimen as extracted DNA and source of specimen and indicate of volume and concentration of the DNA.

Specimen Stability: Frozen (preferred)/Refrigerate/Ambient


Specimen Minimum Volume

Blood, Bone Marrow: 1 mL
Extracted DNA: 100 mcL at 20 ng/mcL concentration

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 14 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

Bone marrow biopsies, slides, paraffin shavings, frozen tissues, paraffin-embedded tissues, paraffin-embedded bone marrow aspirates, moderately to severely clotted, or lithium heparin anticoagulant

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Tuesday, Friday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81450-Targeted genomic sequence analysis panel, hematolymphoid neoplasm or disorder, DNA and RNA when performed, 5-50 genes (eg, BRAF, CEBPA, DNMT3A, EZH2, FLT3, IDH1, IDH2, JAK2, KRAS, KIT, MLL, NRAS, NPM1, NOTCH1), interrogation for sequence variants and copy number variants or rearrangements, or isoform expression of mRNA expression levels, if performed.

Forms

Hematopathology Patient Information (T676) in Special Instructions