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Test Code LABS0691 MISC YPI (FLOW CYTOMETRY) Flow Cytometry

Profile Information

Testing Options Useful For
Leukemia/Lymphoma Phenotyping Aiding in the diagnosis of leukemia and lymphoma, and for post treatment follow up
Platelet Antibodies Differentiating thrombocytopenia of immune origin from non-immune origin by detecting IgG and/or IgM antibodies to platelets
PNH Diagnosing Paroxysmal Nocturnal Hemoglobinuria and monitoring the response to therapy
Reticulated Platelet Differentiating between thrombocytopenic patients with normal or increased megakarypoiesis (such as ITP) and those patients with decreased megakarypoiesis (such as malignancy)
Stem Cell Enumeration (CD34+) Providing an estimate of the number of stem cells capable of repopulating the hematopoietic compartment following high dose chemotherapy
Thrombocytopenia Profile (Platelet Antibody & Reticulated Platelet) An aid in determining the origin of thrombocytopenia (immune, ITP, malignancy)

 

Methodology

Flow Cytometry

Performing Laboratory

Yellowstone Pathology Institute

Specimen Requirements

Specimen Type: Bone Marrow Aspirate

Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions:

  1. Invert several times to mix bone marrow.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Bone Marrow Core

Container/Tube: RPMI

Specimen Volume: 2 cm core length

Specimen Minimum Volume: 1 cm core length

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Peripheral Blood

Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: Sodium Heparin (dark green top), or ACDA (pale yellow, no gel separator)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions:

  1. Invert several times to mix blood.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Fresh Tissue

Container/Tube: RPMI

Specimen Minimum Volume: 2 pieces of tissue 0.2 cm3

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

Specimen Type: Body Fluid/Fine Needle Aspirate

Container/Tube: RPMI

Specimen Volume: Equal parts RPMI and specimen volume

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition and copy of a recent CBC report.

 

Specimen Rejection:

1.  Unlabeled specimens.

2.  Specimens collected in wrong container/tube.

3.  Specimens retained past viability.



Specimen Transport Temperature

Ambient

Specimen Stability

Specimen Type Temperature Time
Varies Ambient

48-72 hours*

*For optimal specimen recovery, transport specimen to YPI immediately.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

88184 Leukemia/Lymphoma Phenotyping (1 marker)

88187 Leukemia/Lymphoma Phenotyping (2-8 markers)

88188 Leukemia/Lymphoma Phenotyping (9-15 markers)

88189 Leukemia/Lymphoma Phenotyping (16+ markers)

86023 Platelet Antibodies

88184, 88185, 88188 PNH Detection

85055 Reticulated Platelet

86367 Stem Cell (CD34+) Enumeration

85055, 86023 Thrombocytopenia Profile