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Test Code LABS0691 MISC YPI (FISH) FISH

Useful For

Detecting genetic abnormalities associated with cancer and other diseases

Profile Information

Testing Options Extended Options
ALL Adult  
ALL Pediatric  
AML Extended  
AML Standard  
AML Favorable-Risk  
High Risk MM With Plasma Cell Enrichment
MM IgH Complex With Plasma Cell Enrichment
MM/MGUS

Reflex to MM IgH Complex if IgH positive

(Only available with global panel,

Tech-Only clients must use the test add-on process.)

With Plasma Cell Enrichment
Plasma Cell Myeloma Risk Stratification (IMWG) With Plasma Cell Enrichment
CLL  
Eosinophilia  
High-Grade/Large B-Cell Lymphoma  
MDS Extended  
MDS Standard  
MPN  
NHL  
XY  

 

Methodology

Fluorescence in situ hybridization (FISH)

Performing Laboratory

Yellowstone Pathology Institute/NeoGenomics

Specimen Requirements

Specimen Type: Bone Marrow Aspirate

Container/Tube:

Preferred: Sodium Heparin (dark green top)

Acceptable: EDTA (lavender top)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions:

  1. Invert several times to mix bone marrow.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition.

Specimen Type: Bone Marrow Core

Container/Tube: RPMI

Specimen Volume: 2 cm core length

Specimen Minimum Volume: 1 cm core length

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition.

Specimen Type: Peripheral Blood

Container/Tube:

Preferred: Sodium Heparin (dark green top)

Acceptable: EDTA (lavender top)

Specimen Volume: 5 mL

Specimen Minimum Volume: 2 mL

Collection Instructions:

  1. Invert several times to mix blood.
  2. Send specimen at ambient temperature in original container.
  3. Include a YPI requisition.

Specimen Type: Fresh Tissue

Container/Tube: RPMI

Specimen Minimum Size: 2 pieces of tissue 0.2 cm3

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition.

Specimen Type: Body Fluid/Fine Needle Aspirate

Container/Tube: RPMI

Specimen Volume: Equal parts RPMI and specimen volume

Collection Instructions: 

  1. Send RPMI specimen at ambient temperature.
  2. Include a YPI requisition.

Specimen Type: FFPE lymph node in block

Container/Tube: Block cassette & 1 H&E cut at 4-5 microns

Specimen Size: 5 microns

Specimen Minimum Size: 4 microns

Collection Instructions: 

  1. Send specimen at ambient temperature.
  2. Area of interest must be circled on H&E when ordering tech-only FISH
  3. Include a YPI requisition.

 

Specimen Type: FFPE lymph node cut slides

Container/Tube: 12 positively charged unstained slides & 1 H&E cut at 4-5 microns

Specimen Size: 12 slides , 5 micron cut

Specimen Minimum Size: 12 slides, 4 micron cut

Collection Instructions: 

  1. Send specimen at ambient temperature.
  2. Area of interest must be circled on H&E when ordering tech-only FISH
  3. Include a YPI requisition.

 

 

Specimen Rejection:

1.  Unlabeled specimens.

2.  Specimens collected in wrong container/tube.

3.  Specimens retained past viability.



Specimen Transport Temperature

Ambient

Specimen Stability

Specimen Type Temperature Time
Varies Ambient

48-72 hours*

*For optimal specimen recovery, transport specimen to YPI immediately.

*MM/MGUS & High Risk MM Panels: May include plasma cell enrichment on specimens of sufficient cellularity. Sample should be received at NeoGenomics Laboratories within 48 hours of collection.

Additional Infomation

Disease State/Panel FISH Probe(s)
ALL BCR/ABL1/ASS1 t(9;22), MLL (11q23)
AML Extended 5q-/-5/+5, 7q-/-7, +8, MLL (11q23), RPN1/MECOM (3q), DEK/CAN t(6;9), ETV6 (12p13), 17p
AML Favorable Risk CBFB (16q22), RUNX1/RUX1T1 (ETO/AML1) t(8;21), PML/RARA t(15;17)
AML M2 RUNX1/RUX1T1 (ETO/AML1) t(8;21)
AML M3 (APL) PML/RARA t(15;17)
AML M4 CBFB (16q22), MLL (11q23)
AML Standard 5q-/-5/+5, 7q-/-7, +8/20q-/-20, MLL (11q23), RUNX1/RUX1T1 (ETO/AML1) t(8;21), PML/RARA t(15;17), CBFB (16q22)
Anaplastic Large Cell Lymphoma ALK for Lymphoma (2p23)
Burkitt Lymphoma MYC/IgH/Cen 8 t(8;14), MYC (8q24)
CLL 6q- [SEC63 (6q21), MYB (6q23)], ATM (11q-); Trisomy 12 (Cen 12); 13q- (13q14, 13q34); CCND1/IgH t(11;14); p53 (17p-)
CML BCR/ABL1/ASS1 t(9;22)
Eosinophilia PDGFRa (4q12), PDGFRb (5q33), FGFR1 (8p12), CBFB (16q22)
Follicular Lymphoma IgH/BCL2 t(14;18)
High-Grade/Large B-Cell Lymphoma BCL6 (3q27), MYC (8q24), IgH/BCL2 t(14;18)
High Risk MM FGFR3/IgH t (4;14), IgH/MAF t(14;16), 13q-/-13, p53 (17p13.1)
Marginal Zone B-Cell Lymphoma/ MALT Lymphoma MALT1 (18q21), API2/MALT1 t(11;18)
MDS Extended 5q-/-5/+5, 7q-/-7, +8/20q-/-20, MLL (11q23), RPN1/MECOM (3q), ETV6 (12p13), 17p-, +19
MDS Standard 5q-/-5/+5, 7q-/-7, +8/20q-/-20, MLL (11q23)
MM IgH Complex CCND1/IgH t(11;14), FGFR3/IgH t(4;14), IgH/MAF t(14;16), Available separately: IgH/MAFB t(14/20).
MM-MGUS 1q+, +3, +9, +5, 13q-/-13, IgH (14q32), p53 (17p13.1)
NHL ALK for Lymphoma (2p23), BCL6 (3q27), MYC (8q24), CCND1/IgH t(11;14), IgH (14q32), IgH/BCL2 t(14;18), MALT1 (18q21)
Plasma Cell Myeloma Risk Stratification (IMWG) FGFR3/IgH t(4;14), IgH/MAF t(14;16), IgH/MAFB t(14;20), 17p- (TP53, CEN 17), Chromosome 1
XY Cen X, DYZ1

Panel configurations are updated periodically. Please check the NeoGenomics website for the most up-to-date information

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

88377 manual

88374 multiplex

88368 (if needed)

88367 (if needed)

additional codes may also be necessary, depending upon initial test results