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Test Code BLOD0477 Thiamine (Vitamin B1), Whole Blood

Additional Codes

Mayo Test Code: TDP

Useful For

Assessment of thiamine deficiency


Thiamine measurement in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type

Whole Blood EDTA

Specimen Required

Patient Preparation: Fasting overnight (12-14 hours). Infants-draw prior to next feeding. Water can be taken as needed.

Supplies: Amber vial (T192)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Invert 8 to 10 times to mix blood.

2. Transfer whole blood into amber vial or tube to protect from light and freeze within 24 hours.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Frozen 14 days

Reject Due To




Mild OK; Gross Reject




Glass vial, clotted specimen, washed erythrocytes non-light protected reject

Reference Values

70-180 nmol/L

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information



If not ordering electronically, complete, print, and send a General Request Form (T239) with the specimen (