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Test Code BLOD0409 Vitamin D, 25-OH, Total

Useful For

  • The quantitative determination of 25-hydroxy vitamin D
  • Assessing vitamin D sufficiency in adults populations in conjunction with other clinical laboratory data.


Enzyme Immunoassay (EIA)

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Serum
Container/Tube: Red top or gold top
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL

Collection Instructions: Send 1 mL serum refrigerated.

Specimen Rejection: Plasma samples will be rejected.

Specimen Transport Temperature


Specimen Stability


Specimen Type Temperature Time
Serum Ambient 24 hours
  Refrigerated 7 days
  Frozen 1 month


Reference Values

30-100 ng/mL (normal)


 < 20 ng/ml = deficient   

20-29 ng/ml = insufficient  

30-100 ng/ml = sufficient  

> 100 ng/ml = potential intoxication

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding