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Test Code NBLD0229 Respiratory Syncytial Virus (RSV) Antigen, Rapid

Methodology

Indirect Immunofluorescence

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Note: Samples must be from a patient less than 20 years of age.  Call Bozeman Health Laboratory Services at 406-414-5010 to obtain a VTM kit and NP swab. Good specimen quality is key to accurate test results.

 

Specimen source is required

Submit one of the following specimens:

 

Specimen Type: Nasopharyngeal (NP) swab

Collection Container/Tube: NP swab

Submission Container/Tube:  Viral transport media (ETM)

 

Specimen Volume: Entire collected specimen

Collection Instructions:

  1. Tilt patient’s head at a 70° angle.
  2. Holding swab between thumb and forefinger of 1 hand and sheath in other hand, give soft wire a 10° to 20° bend.
  3. Press swab tip on mucosal surface of mid-inferior portion of inferior turbinate, and rub swab tip several times across mucosal surface to loosen and collect cellular material (viruses adhere to epithelial cells, this step is critical). Eyes may tear momentarily.
  4. Slowly rotate swab as it is being withdrawn and repeat procedure for second nostril (same swab). This technique will deliver the optimal specimen.
  5. Return swab to viral transport media tube.
  6. Carefully break swab shaft at scoreline. Use care to avoid splashing of contents.
  7. Label tube with patient’s name (first and last), date and actual time of collection, and type of specimen.

Specimen Type: Nasal wash

Container/Tube: Tube of viral transport media

 

Specimen Volume: Entire collected  specimen

Collection Instructions:

  1. Tilt patient’s head at a 70° angle.
  2. Fill a rubber bulb with 2 mL to 3 mL of sterile saline.
  3. Insert end of rubber bulb through a nostril and into nasal cavity.  Instill saline and immediately recover it by aspiration.
  4. Transfer specimen to tube of viral transport media.
  5. Label tube with patient’s name (first and last), date and actual time of collection, and type of specimen

Specimen Transport Temperature

Ambient <1 hour/Refrigerated >1 hour

Specimen Stability

 

Specimen Type Temperature Time
NP sample in Viral Transport Media (VTM) Ambient 1 hour
  Refrigerated 72 hours

 

Reference Values

Negative:  Negative for the presence of RSV antigen

 

Positive:  A positive result indicates the presence of RSV antigen

Critical Value (automatic call-back): Positive

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

87807