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Test Code BLOD0923 Direct Coombs

Useful For

Diagnosing hemolytic disease of the newborn, hemolytic anemia, autoimmune processes (either acquired or drug-related), and the evaluation of possible transfusion reactions.


Capture solid phase automation and manual blood banking methods

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Whole blood

Container/Tube: Pink top (K2 EDTA), Purple top (EDTA), or Cord Blood red top

Specimen Volume: 6 mL

Specimen Pediatric Volume: 1 full, lavender-top (EDTA) Microtainer

Collection Instructions: Do not centrifuge. Forward unprocessed whole blood promptly.

Additional Information:  Include report of diagnosis and history of transfusions and drug therapy.

Specimen Rejection: Gel tube

Specimen Transport Temperature

Ambient <24 hours/Refrigerated >24 hours

Specimen Stability


Specimen Type Temperature Time
Whole Blood EDTA Ambient 24 hours
  Refrigerated 10 days


Reference Values

Negative (reported as positive or negative)

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

86880-Coombs, direct