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Test Code BLOD0840 ENA (Extractable Nuclear Antigen) Panel 3

Useful For

Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive


Enzyme-linked fluorescent immunoasay (EliA)

Profile Information


Test Code Test Name Test Components




Sjögren’s Syndrome Antibodies, IgG SS-A/Ro Antibodies, IgG Yes
SS-B/La Antibodies, IgG Yes
BLOD1380 Sm (Smith D) Antibodies, IgG   Yes
BLOD1381 RNP Antibodies, IgG   Yes
BLOD0775 DNA Double-Stranded Antibody, IgG   Yes
BLOD0308 Scleroderma Antibodies (SCL-70), IgG   Yes
BLOD0306 Jo-1 Antibodies, IgG   Yes


Performing Laboratory

Bozeman Outpatient Services at North 19th

Specimen Requirements

Specimen Type: Serum
Container/Tube: Gold top or Red top

Specimen Volume: 3 ml serum 

Specimen Minimum Volume: 2 mL serum

Collection Instructions:  Allow time for specimen to clot.  Spin down within 2 hours of collection.

Specimen Rejection: 

​1.  Grossly hemolyzed or lipemic samples will be rejected. 

2.  Repeatedly freezing and thawing samples can cause analyte deterioration. Thaw only once.


Specimen Transport Temperature

Ambient <8 hours/Refrigerated <48 hours/Frozen >48 hours

Specimen Stability


Specimen Type Temperature Time
Serum Ambient 8 hours
  Refrigerated 48 hours
  Frozen 30 days


Reference Values

SS-A/Ro IgG:

<7.0 U/mL (negative)

7.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)


SS-B/La IgG:

<7.0 U/mL (negative)

7.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)


Sm (Smith D) IgG:

<7.0 U/mL (negative)

7.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)



<5.0 U/mL (negative)

5.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)



<10.0 IU/mL (negative)

10.0-15.0 IU/mL (equivocal)

>15.0 IU/mL (positive)


Scleroderma SCL-70 IgG:

<7.0 U/mL (negative)

7.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)


Jo-1 IgG:

<7.0 U/mL (negative)

7.0-10.0 U/mL (equivocal)

>10.0 U/mL (positive)



Day(s) Test Set Up

Monday, Wednesday, Friday

Test Classification and CPT Coding