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Test Code BLOD0563 Lactic Acid, Plasma

Important Note

Special handling required.  Refer to Specimen Requirements.

Useful For

Evaluating metabolic acidosis, dehydration, diabetic acidosis, and tissue hypoxia


Lactate to Pyruvate/NAD to NADH/Calibrated/37° C/Bichromatic end point

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Specimen Type: Plasma

Collection Container/Tube: Grey top (potassium oxalate/sodium fluoride)

Submission Container/Tube: Plastic tube

Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL

Collection Instructions:

1. Draw blood from a fasting patient without the use of a tourniquet.

2. Immediately place tube on wet ice.

3. Spin down within 15 minutes of draw, separate plasma from cells, transfer plasma to plastic tube, and place tube on wet ice.

Additional Information: Intravenous injection of epinephrine, glucose, bicarbonate, or other infusions that modify the acid-base balance causes elevation of lactate not necessarily related to hypoxia.


Specimen Rejection: Grossly hemolyzed or icteric specimen

Specimen Transport Temperature

On wet ice

Specimen Stability


Specimen Type Temperature Time

Whole blood (potassium oxalate/sodium fluoride)

On ice 15 minutes

Plasma, spun and separated

(potassium oxalate/sodium fluoride)

Ambient 8 hours
  Refrigerated 14 days
  Frozen 1 month


Reference Values

0.4-2.0 mmol/L

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding