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Test Code BLOD0559 Glucose 2 Hour Post Prandial

Useful For

A screening test for gestational diabetes

Methodology

Hexokinase/Ultraviolet (UV)/Bichromatic End point

Performing Laboratory

Bozeman Health Laboratory Services

Specimen Requirements

Patient Preparation:

1. Patient should maintain a normal carbohydrate diet (at least 300 g of carbohydrates per day) for 3 days prior to test. 
2. Patient should follow their physicians instructions in regards to fasting.

3. Patient must not eat or drink during testing. Ice chips may be given to moisten mouth.

 

Performance of Test:

  1. Have adult patient drink entire 75 g of glucose tolerance beverage.
  2. Ingestion should be completed within 10 minutes. Timing starts at this point.
  3. Exactly 2 hours after ingestion of glucose beverage, draw blood specimen.

Specimen Collection:

Specimen Type: Plasma or serum
Container/Tube: Light-green top (lithium heparin gel), red top, gold top, or grey top (potassium oxalate/sodium fluoride)
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL

Collection Instructions:

1. If there is a delay in transport of >8 hours, refrigerate specimen, and send specimen refrigerated.

2. Label specimen as plasma or serum and with time of draw.

 

Specimen Transport Temperature

Plasma or serum-Ambient <8 hours/Refrigerated >8 hours

 

Specimen Stability

 

Specimen Type Temperature Time
Heparinized plasma or serum Ambient 8 hours
  Refrigerated

3 days

  Frozen 1 month
Sodium Fluoride plasma Ambient 3 days

Note: Glycolysis decreases serum glucose by approximately 5% to 7% per hour in normal, uncentrifuged, coagulated blood at ambient temperature.

Reference Values

Normal: 70-140 mg/dL

Pre-diabetes: 140-200 mg/dL

Probable diabetes: >200 mg/dL

Critical values (automatic call-back): ≤50 mg/dL or ≥400 mg/dL

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

82947-Glucose, quantitative